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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | F263 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | F258 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY | - |
dc.citation.volume | 260 | - |
dc.contributor.author | Kwon, H. Moo | - |
dc.contributor.author | Yamauchi, Atsushi | - |
dc.contributor.author | Uchida, Shinichi | - |
dc.contributor.author | Robey, Robert Brooks | - |
dc.contributor.author | Garcia-Perez, Arlyn | - |
dc.contributor.author | Burg., Maurice B. | - |
dc.contributor.author | Handler, Joseph S. | - |
dc.date.accessioned | 2023-12-22T13:08:42Z | - |
dc.date.available | 2023-12-22T13:08:42Z | - |
dc.date.created | 2014-06-03 | - |
dc.date.issued | 1991-02 | - |
dc.description.abstract | Canine renal cells in culture (MDCK cells) accumulate organic osmolytes, including myo-inositol (MI), in response to hypertonic stress. When medium tonicity is increased, intracellular concentration of MI rises because hypertonicity elicits increased uptake of MI via Na-MI cotransporter(s). To study the mechanism for this increase in cotransporter activity, poly(A)+ RNA isolated from MDCK cells maintained in hypertonic or isotonic medium was injected into Xenopus oocytes, and Na-dependent MI uptake was measured 3-5 days later. Poly(A)+ RNA from hypertonic cells induced clear expression of the cotransporter. In contrast, oocytes injected with poly(A)+ RNA isolated from MDCK cells maintained in isotonic medium exhibited cotransporter activity like oocytes injected with water. Upon size fractionation of RNA, peak activity appeared in a fraction that contained poly(A)+ RNA with median size of ~4 kilobases. Na-dependent MI uptake by poly(A)+ RNA-injected oocytes was inhibited by both phlorizin and phloretin. We suggest that hypertonicity-induced upregulation of the Na-MI cotransporter involves an increase in mRNA and synthesis of cotransporter protein(s). | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY, v.260, no.2, pp.F258 - F263 | - |
dc.identifier.issn | 0002-9513 | - |
dc.identifier.scopusid | 2-s2.0-0025979643 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4919 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025979643 | - |
dc.identifier.wosid | A1991EY85600087 | - |
dc.language | 영어 | - |
dc.publisher | American Physiological Society | - |
dc.title | Renal Na-myo-inositol cotransporter mRNA expression in Xenopus oocytes: Regulation by hypertonicity | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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