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DC Field | Value | Language |
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dc.citation.endPage | 20621 | - |
dc.citation.number | 32 | - |
dc.citation.startPage | 20615 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 273 | - |
dc.contributor.author | Rim, JS | - |
dc.contributor.author | Atta, MG | - |
dc.contributor.author | Dahl, SC | - |
dc.contributor.author | Berry, GT | - |
dc.contributor.author | Handler, JS | - |
dc.contributor.author | Kwon, H. Moo | - |
dc.date.accessioned | 2023-12-22T12:35:56Z | - |
dc.date.available | 2023-12-22T12:35:56Z | - |
dc.date.created | 2014-06-05 | - |
dc.date.issued | 1998-08 | - |
dc.description.abstract | he sodium/myo-inositol cotransporter is a plasma membrane protein responsible for concentrative cellular accumulation of myo-inositol in a variety of tissues. When cells in kidney and brain are exposed to a hyperosmolar salt condition (hypertonicity) due to the operation of urinary concentration mechanism and pathological conditions, respectively; they survive the stress of hypertonicity by raising the cellular concentration of myo-inositol. Transcription of the sodium/myo-inositol cotransporter gene is markedly stimulated in response to hypertonicity, leading to an increase in the activity of the cotransporter, which in turn drives the osmoprotective accumulation of myo-inositol. To understand the molecular mechanisms by which hypertonicity stimulates transcription, we analyzed the BI-flanking region of the cotransporter gene for cis-acting regulatory sequences. We identified five tonicity-responsive enhancers that are scattered over 50 kilobase pairs. All the enhancers are variations of the same type of enhancer interacting with the transcription factor named tonicity-responsive enhancer binding protein. In vivo methylation experiments demonstrated that exposure of cells to hypertonicity increases the binding of tonicity-responsive enhancer binding protein to the enhancer sites, indicating that all of these enhancers are involved in the transcriptional stimulation. We conclude that the sodium/myo-inositol cotransporter gene is regulated by a large region (similar to 50 kilobase pairs) upstream of the gene. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.32, pp.20615 - 20621 | - |
dc.identifier.doi | 10.1074/jbc.273.32.20615 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0032493754 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4908 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032493754 | - |
dc.identifier.wosid | 000075305400096 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Transcription of the sodium/myo-inositol cotransporter gene is regulated by multiple tonicity-responsive enhancers spread over 50 kilobase pairs in the 5 '-flanking region | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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