Full metadata record
DC Field | Value | Language |
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dc.citation.endPage | 284 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 269 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 49 | - |
dc.contributor.author | Kang, Hyun Je | - |
dc.contributor.author | Cheon, Na Young | - |
dc.contributor.author | Park, Hyun | - |
dc.contributor.author | Jeong, Gyu Won | - |
dc.contributor.author | Ye, Byeong Jin | - |
dc.contributor.author | Yoo, Eun Jin | - |
dc.contributor.author | Lee, Jun Ho | - |
dc.contributor.author | Hur, Jin-Hoe | - |
dc.contributor.author | Lee, Eun-A | - |
dc.contributor.author | Kim, Hongtae | - |
dc.contributor.author | Lee, Kyoo-young | - |
dc.contributor.author | Choi, Soo Youn | - |
dc.contributor.author | Lee-Kwon, Whaseon | - |
dc.contributor.author | Myung, Kyungjae | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.contributor.author | Kwon, Hyug Moo | - |
dc.date.accessioned | 2023-12-21T16:36:43Z | - |
dc.date.available | 2023-12-21T16:36:43Z | - |
dc.date.created | 2020-12-13 | - |
dc.date.issued | 2021-01 | - |
dc.description.abstract | R-loops are three-stranded, RNA–DNA hybrid, nucleic acid structures produced due to inappropriate processing of newly transcribed RNA or transcription-replication collision (TRC). Although R-loops are important for many cellular processes, their accumulation causes genomic instability and malignant diseases, so these structures are tightly regulated. It was recently reported that R-loop accumulation is resolved by methyltransferase-like 3 (METTL3)-mediated m6A RNA methylation under physiological conditions. However, it remains unclear how R-loops in the genome are recognized and induce resolution signals. Here, we demonstrate that tonicity-responsive enhancer binding protein (TonEBP) recognizes R-loops generated by DNA damaging agents such as ultraviolet (UV) or camptothecin (CPT). Single-molecule imaging and biochemical assays reveal that TonEBP preferentially binds a R-loop via both 3D collision and 1D diffusion along DNA in vitro. In addition, we find that TonEBP recruits METTL3 to R-loops through the Rel homology domain (RHD) for m6A RNA methylation. We also show that TonEBP recruits RNaseH1 to R-loops through a METTL3 interaction. Consistent with this, TonEBP or METTL3 depletion increases R-loops and reduces cell survival in the presence of UV or CPT. Collectively, our results reveal an R-loop resolution pathway by TonEBP and m6A RNA methylation by METTL3 and provide new insights into R-loop resolution processes. | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.49, no.1, pp.269 - 284 | - |
dc.identifier.doi | 10.1093/nar/gkaa1162 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-85099721455 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/48878 | - |
dc.identifier.url | https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa1162/6031430 | - |
dc.identifier.wosid | 000610552100026 | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | TonEBP recognizes R-loops and initiates m6A RNA methylation for R-loop resolution | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | HYBRIDS | - |
dc.subject.keywordPlus | THREATS | - |
dc.subject.keywordPlus | REPAIR | - |
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