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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability

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MYOINOSITOL AND BETAINE TRANSPORTERS REGULATED BY TONICITY ARE BASOLATERAL IN MDCK CELLS

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dc.contributor.author YAMAUCHI, A ko
dc.contributor.author Kwon, H. Moo ko
dc.contributor.author UCHIDA, S ko
dc.contributor.author PRESTON, AS ko
dc.contributor.author HANDLER, JS ko
dc.date.available 2014-06-03T00:54:48Z -
dc.date.created 2014-06-02 ko
dc.date.issued 1991-07 ko
dc.identifier.citation AMERICAN JOURNAL OF PHYSIOLOGY, v.261, no.1, pp.F197 - F202 ko
dc.identifier.issn 0002-9513 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/4865 -
dc.description.abstract Myo-inositol and glycinebetaine are compatible osmolytes accumulated in the renal medulla and in MDCK cells cultured in hypertonic media. Both osmolytes are taken up by MDCK cells on Na-coupled transporters. The maximal velocity (V(max)) of both cotransporters is increased by culture in hypertonic medium. When hypertonic MDCK cells are shifted to isotonic medium there is a large transient efflux of osmolytes. To determine the polarity of the cotransporters and the transient efflux, we grew MDCK cells on a porous support to assay transport separately at their apical and basolateral surfaces. In hypertonic cells, basolateral uptake of both osmolytes was 1) more than 10-fold apical uptake, 2) > 96% Na dependent, 3) 25- (myo-inositol) and 16-fold (glycinebetaine) uptake in isotonic cells, reaching a maximum 24 h after the switch to hypertonic medium. When medium osmolarity was decreased from hypertonic to isotonic, myo-inositol uptake reversed to the isotonic level within 1 day; glycinebetaine uptake decreased more slowly. When medium osmolarity was decreased from hypertonic to isotonic, there was a large transient increase in basolateral efflux of both osmolytes. ko
dc.description.statementofresponsibility open -
dc.language 영어 ko
dc.publisher American Physiological Society ko
dc.title MYOINOSITOL AND BETAINE TRANSPORTERS REGULATED BY TONICITY ARE BASOLATERAL IN MDCK CELLS ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0025874198 ko
dc.identifier.wosid A1991FY35700096 ko
dc.type.rims ART ko
dc.description.wostc 112 *
dc.date.tcdate 2015-05-06 *
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025874198 ko
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