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Kang, Joo H.
Translational Multiscale Biofluidics Lab (TMB Lab)
Research Interests
  • Biomedical devices, infectious disease, organ-on-a-chip, microfluidics, mechanobiology

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An inflammatory vascular endothelium-mimicking microfluidic device to enable leukocyte rolling and adhesion for rapid infection diagnosis

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Title
An inflammatory vascular endothelium-mimicking microfluidic device to enable leukocyte rolling and adhesion for rapid infection diagnosis
Author
Kwon, SeyongKurmashev, AmanzholLee, Min SeokKang, Joo H.
Issue Date
2020-11
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.168, pp.112558
Abstract
Recruitment of circulating leukocytes to sites of infection is of utmost importance in the development, propagation, and outcome of sepsis. These multi-step processes are mediated by interactions between adhesion receptors of leukocytes and cell adhesion molecules (CAMs) of endothelial cells, such as P-selectin, E-selectin and ICAM-1. However, the potential utility of the CAMs-facilitated leukocyte capture has not been thoroughly investigated as an index of the host response to infection for diagnostic purposes. Here, we report that the systemic infection affects the expression of CAMs ligands on leukocytes, upregulating the expression of P-selectin ligand-1 (PSGL-1) and increasing the number of PSGL-1- and E-selectin ligand-1 (ESL-1)-expressing leukocyte levels in septic blood. We leveraged this finding to determine infection by measuring the increased adhesion of leukocytes to an inflammatory vascular endothelium-mimicking microchannel coated with CAMs. We successfully validated that the proposed method can significantly differentiate infection in bacteremia and endotoxemia models in rats as early as an hour post-infection using a finger-prick volume of blood (50 mu L), which were unachievable with the conventional diagnostic methods.
URI
https://scholarworks.unist.ac.kr/handle/201301/48570
URL
https://www.sciencedirect.com/science/article/pii/S0956566320305509?via%3Dihub
DOI
10.1016/j.bios.2020.112558
ISSN
0956-5663
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BME_Journal Papers
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