13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, pp.1722 - 1724
Abstract
This work presents microfluidic chemical interface systems enabling the active modulation of synthetic microbial signaling pathways by controlling the diffusion and reaction of cell-signaling molecules by directly activating and inactivating gene expression in an initially homogenous population of cells. The strategy is to combine a microfluidic system that acts as a distributed inhibitor system and a synthetic gene circuit that contains a positive feedback loop activated by acyl-homoserine lactone (AHL) to form synthetic microbial patterns; the activator molecules are AHL and the inhibitors are hydroxyl ions.
Publisher
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009