dc.citation.conferencePlace |
KO |
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dc.citation.title |
대한화학회 118회 학술발표회 |
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dc.contributor.author |
최봉서 |
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dc.contributor.author |
최혁준 |
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dc.contributor.author |
강세병 |
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dc.date.accessioned |
2023-12-19T20:07:21Z |
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dc.date.available |
2023-12-19T20:07:21Z |
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dc.date.created |
2017-01-03 |
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dc.date.issued |
2016-10-12 |
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dc.description.abstract |
We construct a novel recombinant secondary antibody mimic, GST-ABD, which can bind to the Fc regions of target-bound primary antibodies and acquire multiple HRPs simultaneously. We produce it in tenth of mg quantities with a bacterial overexpression system and simple purification procedures, significantly reducing the manufacturing cost and time without the use of animals. GST-ABD is effectively conjugated with 3 HRPs per molecule on an average and selectively bind to the Fc region of primary antibodies derived from three different species (mouse, rabbit, and rat). HRP-conjugated GST-ABD (HRP-GST-ABD) is successfully used as an alternative to secondary antibodies to amplify target-specific signals in both ELISA and immunohistochemistry regardless of the target molecules and origin of primary antibodies used. GST-ABD also successfully serves as an anchoring adaptor on the surface of GSH-coated plates for immobilizing antigen-capturing antibodies in an orientation-controlled manner for sandwich-type indirect ELISA through simple molecular recognition without any complicated chemical modification. |
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dc.identifier.bibliographicCitation |
대한화학회 118회 학술발표회 |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/39903 |
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dc.language |
영어 |
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dc.publisher |
대한화학회 |
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dc.title |
A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2016-10-12 |
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