Induction of pulmonary mucosal immune responses with a protein vaccine targeted to the DEC-205/CD205 receptor
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- Induction of pulmonary mucosal immune responses with a protein vaccine targeted to the DEC-205/CD205 receptor
- Do, Yoonkyung; Didierlaurent, Arnaud M.; Ryu, Seongho; Koh, Hyein; Park, Chae Gyu; Park, Steven; Perlin, David S.; Powell, Bradford S.; Steinman, Ralph M.
- CD205/DEC-205; Cellular immunity; Dendritic cells; LcrV; Mucosal; Y. pestis
- Issue Date
- ELSEVIER SCI LTD
- VACCINE, v.30, no.45, pp.6359 - 6367
- It is of great interest to develop a pneumonic plague vaccine that would induce combined humoral and cellular immunity in the lung. Here we investigate a novel approach based on targeting of dendritic cells using the DEC-205/CD205 receptor (DEC) via the intranasal route as way to improve mucosal cellular immunity to the vaccine. Intranasal administration of Yersinia pestis LcrV (V) protein fused to anti-DEC antibody together with poly IC as an adjuvant induced high frequencies of IFN-gamma secreting CD4(+) T cells in the airway and lung as well as pulmonary IgG and IgA antibodies. Anti-DEC:LcrV was more efficient to induce IFN-gamma/TNF-alpha/IL-2 secreting polyfunctional CD4(+) T cells when compared to non-targeted soluble protein vaccine. In addition, the intranasal route of immunization with anti-DEC:LcrV was associated with improved survival upon pulmonary challenge with the virulent CO92 Y. pestis. Taken together, these data indicate that targeting dendritic cells via the mucosal route is a potential new avenue for the development of a mucosal vaccine against pneumonic plague.
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