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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics
  • Bio-Nanotechnology
  • Biofluids
  • Biomaterials
  • Tissue Engineering and Regenerative Medicine.

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Microprinted Feeder Cells Guide Embryonic Stem Cell Fate

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Title
Microprinted Feeder Cells Guide Embryonic Stem Cell Fate
Author
Tavana, HosseinMosadegh, BobakZamankhan, ParsaGrotberg, James B.Takayama, Shuichi
Keywords
Aqueous two phase system; Cell printing; Cell-cell contact; Embryonic stem cells; Microenvironments
Issue Date
2011-10
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.108, no.10, pp.2509 - 2516
Abstract
We introduce a non-contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are printed on a substrate residing within a bath of the immersion aqueous phase. Due to their affinity to the denser phase, cells remain localized within the drops and adhere to regions of the substrate underneath the drops. We show the utility of this technology for creating duplex heterocellular stem cell niches by printing two different support cell types on a gel surface and overlaying them with mouse embryonic stem cells (mESCs). As desired, the type of printed support cell spatially direct the fate of overlaid mESCs. Interestingly, we found that interspaced mESCs colonies on differentiation-inducing feeder cells show enhanced neuronal differentiation and give rise to dense networks of neurons. This cell printing technology provides unprecedented capabilities to efficiently identify the role of various feeder cells in guiding the fate of stem cells
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DOI
10.1002/bit.23190
ISSN
0006-3592
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BME_Journal Papers
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