File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

이현우

Rhee, Hyun-Woo
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging

Author(s)
Rhee, Hyun-WooZou, PengUdeshi, Namrata D.Martell, Jeffrey D.Mootha, Vamsi K.Carr, Steven A.Ting, Alice Y.
Issued Date
2013-03
DOI
10.1126/science.1230593
URI
https://scholarworks.unist.ac.kr/handle/201301/3482
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84874956967
Citation
SCIENCE, v.339, no.6125, pp.1328 - 1331
Abstract
Microscopy and mass spectrometry (MS) are complementary techniques: The former provides spatiotemporal information in living cells, but only for a handful of recombinant proteins at a time, whereas the latter can detect thousands of endogenous proteins simultaneously, but only in lysed samples. Here, we introduce technology that combines these strengths by offering spatially and temporally resolved proteomic maps of endogenous proteins within living cells. Our method relies on a genetically targetable peroxidase enzyme that biotinylates nearby proteins, which are subsequently purified and identified by MS. We used this approach to identify 495 proteins within the human mitochondrial matrix, including 31 not previously linked to mitochondria. The labeling was exceptionally specific and distinguished between inner membrane proteins facing the matrix versus the intermembrane space (IMS). Several proteins previously thought to reside in the IMS or outer membrane, including protoporphyrinogen oxidase, were reassigned to the matrix by our proteomic data and confirmed by electron microscopy. The specificity of peroxidase-mediated proteomic mapping in live cells, combined with its ease of use, offers biologists a powerful tool for understanding the molecular composition of living cells.
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
ISSN
0036-8075
Keyword
PROTOPORPHYRINOGEN OXIDASECROSS-LINKHEMEPEROXIDASEBIOTINYLATIONBIOSYNTHESISMICROSCOPYTYRAMIDEDISEASECOMPLEX

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.