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Lee, Changha
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Role of Reactive Oxygen Species in Escherichia coli Inactivation by Cupric Ion

Author(s)
Park, Hee-JinNguyen, Thuy T. M.Yoon, JeyongLee, Changha
Issued Date
2012-10
DOI
10.1021/es302379q
URI
https://scholarworks.unist.ac.kr/handle/201301/3408
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84870044743
Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.46, no.20, pp.11299 - 11304
Abstract
This study demonstrated Escherichia coil inactivation by cupric ion (Cu[II]), focusing on intracellular generation and consumption of reactive oxygen species (ROS) including superoxide and hydroxyl radials. In the presence of Cu(II), intracellular superoxide levels of E. coil decreased in a concentration-dependent manner, indicating that superoxide radical was used to reduce Cu(II) to Cu(I) in cells. The variation in the hydroxyl radical level by adding Cu(II) was negligible. Molecular oxygen and hydroxyl radical scavengers did not affect the inactivation efficacy of E. coil by Cu(II), excluding the possibility that hydroxyl radicals induced by the copper-mediated reduction of oxygen contributed to the microbiocidal action of Cu(II). However, the inactivation of E. coil by Cu(II) was considerably inhibited and accelerated by a Cu(I)-chelating agent and a Cu(II)-reducing agent, respectively. Our results suggest that the rnicrobiocidal action of Cu(II) is attributable to the cytotoxicity of cellularly generated Cu(I), which does not appear to be associated with oxidative damage by Cu(I)-driven ROS.
Publisher
AMER CHEMICAL SOC
ISSN
0013-936X
Keyword
TIO2 PHOTOCATALYTIC DISINFECTIONCOPPER TOXICITYOXIDATIVE STRESSSILVER IONSE. COLISUPEROXIDEGENERATIONRESISTANCEIRONMECHANISMS

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