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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics

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Title
Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
Author
Kim, Chi-JuKi, Dong YeobPark, JuheeSunkara, VijayaKim, Tae-HyeongMin, YooHongCho, Yoon-Kyoung
Issue Date
2020-03
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.20, no.5, pp.949 - 957
Abstract
Platelets play crucial roles in hemostasis and immunity. Over the last decades, clinical evidence has revealed the significance of platelets as complementary biomarkers for the detection and treatment of various diseases, including cancer. Due to a lack of well standardized convenient isolation methods for platelets, pre-analytical factors such as complex handling procedures negatively impact the quality of the platelet samples, including overactivation, low purity, and poor reproducibility. This may lead to biased interpretation of various downstream analyses, such as proteomic and genomic analyses. Herein, we describe a fully automated lab-on-a-disc-based method of platelet isolation from a small volume of blood (<1 mL). This method provides higher yields (>4 folds) and purity (>99%) and lower platelet activation than the conventional method. Moreover, it was also superior in the detection of platelet-related RNAs CD41, PF4, and P2Y12 due to lower contamination with white blood cells.
URI
https://scholarworks.unist.ac.kr/handle/201301/31969
URL
https://pubs.rsc.org/en/content/articlelanding/2020/LC/C9LC01140D#!divAbstract
DOI
10.1039/c9lc01140d
ISSN
1473-0197
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