Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
Cited 0 times inCited 0 times in
- Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
- Kim, Chi-Ju; Ki, Dong Yeob; Park, Juhee; Sunkara, Vijaya; Kim, Tae-Hyeong; Min, YooHong; Cho, Yoon-Kyoung
- Issue Date
- ROYAL SOC CHEMISTRY
- LAB ON A CHIP, v.20, no.5, pp.949 - 957
- Platelets play crucial roles in hemostasis and immunity. Over the last decades, clinical evidence has revealed the significance of platelets as complementary biomarkers for the detection and treatment of various diseases, including cancer. Due to a lack of well standardized convenient isolation methods for platelets, pre-analytical factors such as complex handling procedures negatively impact the quality of the platelet samples, including overactivation, low purity, and poor reproducibility. This may lead to biased interpretation of various downstream analyses, such as proteomic and genomic analyses. Herein, we describe a fully automated lab-on-a-disc-based method of platelet isolation from a small volume of blood (<1 mL). This method provides higher yields (>4 folds) and purity (>99%) and lower platelet activation than the conventional method. Moreover, it was also superior in the detection of platelet-related RNAs CD41, PF4, and P2Y12 due to lower contamination with white blood cells.
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.