hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms

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Title
hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms
Author
Kwon, Paul KwanghoLee, Kyung-HaKim, Ji-hyungTae, SookilHam, SeokjinJeong, Young-HunKim, Sung WookKang, ByungheeKim, Hyo-MinChoi, Jung-HyunYi, HeeKu, Hyun-OkRoh, Tae-YoungLim, ChunghunKim, Kyong-Tai
Issue Date
2020-02
Publisher
American Society for Microbiology
Citation
MOLECULAR AND CELLULAR BIOLOGY, v.40, no.6, pp.e00537-19
Abstract
Circadian gene expression is defined by the gene-specific phase and amplitude of daily oscillations in mRNA and protein levels. D site-binding protein mRNA (Dbp mRNA) shows high-amplitude oscillation; however, the underlying mechanism remains elusive. Here, we demonstrate that heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a key regulator that activates Dbp transcription via the poly(C) motif within its proximal promoter. Biochemical analyses identified hnRNP K as a specific protein that directly associates with the poly(C) motif in vitro. Interestingly, we further confirmed the rhythmic binding of endogenous hnRNP K within the Dbp promoter through chromatin immunoprecipitation as well as the cycling expression of hnRNP K. Finally, knockdown of hnRNP K decreased mRNA oscillation in both Dbp and Dbp-dependent clock genes. Taken together, our results show rhythmic protein expression of hnRNP K and provide new insights into its function as a transcriptional amplifier of Dbp.
URI
https://scholarworks.unist.ac.kr/handle/201301/31563
URL
https://mcb.asm.org/content/40/6/e00537-19
DOI
10.1128/mcb.00537-19
ISSN
0270-7306
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SLS_Journal Papers
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