hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms
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- hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms
- Kwon, Paul Kwangho; Lee, Kyung-Ha; Kim, Ji-hyung; Tae, Sookil; Ham, Seokjin; Jeong, Young-Hun; Kim, Sung Wook; Kang, Byunghee; Kim, Hyo-Min; Choi, Jung-Hyun; Yi, Hee; Ku, Hyun-Ok; Roh, Tae-Young; Lim, Chunghun; Kim, Kyong-Tai
- Issue Date
- American Society for Microbiology
- MOLECULAR AND CELLULAR BIOLOGY, v.40, no.6, pp.e00537-19
- Circadian gene expression is defined by the gene-specific phase and amplitude of daily oscillations in mRNA and protein levels. D site-binding protein mRNA (Dbp mRNA) shows high-amplitude oscillation; however, the underlying mechanism remains elusive. Here, we demonstrate that heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a key regulator that activates Dbp transcription via the poly(C) motif within its proximal promoter. Biochemical analyses identified hnRNP K as a specific protein that directly associates with the poly(C) motif in vitro. Interestingly, we further confirmed the rhythmic binding of endogenous hnRNP K within the Dbp promoter through chromatin immunoprecipitation as well as the cycling expression of hnRNP K. Finally, knockdown of hnRNP K decreased mRNA oscillation in both Dbp and Dbp-dependent clock genes. Taken together, our results show rhythmic protein expression of hnRNP K and provide new insights into its function as a transcriptional amplifier of Dbp.
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