Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

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Title
Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate
Author
Hwang, Ho WonYoon, JiheeMin, KyoungseonKim, Min-SikKim, Seung-JinCho, Dae HaengSusila, HadiyatiNa, Jeong-GeolOh, Min-KyuKim, Yong Hwan
Issue Date
2020-06
Publisher
ELSEVIER SCIENCE SA
Citation
CHEMICAL ENGINEERING JOURNAL, v.389, pp.124394
Abstract
Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H2. The conversion yield and specificity were close to 100% when the strain’s energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.
URI
https://scholarworks.unist.ac.kr/handle/201301/31264
URL
https://www.sciencedirect.com/science/article/pii/S1385894720303855
DOI
10.1016/j.cej.2020.124394
ISSN
1385-8947
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ECHE_Journal Papers
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