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DC Field | Value | Language |
---|---|---|
dc.citation.number | 8 | - |
dc.citation.startPage | e1002238 | - |
dc.citation.title | PLOS GENETICS | - |
dc.citation.volume | 7 | - |
dc.contributor.author | Rutkowski, Rachael | - |
dc.contributor.author | Dickinson, Robin | - |
dc.contributor.author | Stewart, Graeme | - |
dc.contributor.author | Craig, Ashley | - |
dc.contributor.author | Schimpl, Marianne | - |
dc.contributor.author | Keyse, Stephen M. | - |
dc.contributor.author | Gartner, Anton | - |
dc.date.accessioned | 2023-12-22T06:06:31Z | - |
dc.date.available | 2023-12-22T06:06:31Z | - |
dc.date.created | 2020-01-30 | - |
dc.date.issued | 2011-08 | - |
dc.description.abstract | Maintaining genome stability in the germline is thought to be an evolutionarily ancient role of the p53 family. The sole Caenorhabditis elegans p53 family member CEP-1 is required for apoptosis induction in meiotic, late-stage pachytene germ cells in response to DNA damage and meiotic recombination failure. In an unbiased genetic screen for negative regulators of CEP-1, we found that increased activation of the C. elegans ERK orthologue MPK-1, resulting from either loss of the lip-1 phosphatase or activation of let-60 Ras, results in enhanced cep-1-dependent DNA damage induced apoptosis. We further show that MPK-1 is required for DNA damage-induced germ cell apoptosis. We provide evidence that MPK-1 signaling regulates the apoptotic competency of germ cells by restricting CEP-1 protein expression to cells in late pachytene. Restricting CEP-1 expression to cells in late pachytene is thought to ensure that apoptosis doesn't occur in earlier-stage cells where meiotic recombination occurs. MPK-1 signaling regulates CEP-1 expression in part by regulating the levels of GLD-1, a translational repressor of CEP-1, but also via a GLD-1-independent mechanism. In addition, we show that MPK-1 is phosphorylated and activated upon ionising radiation (IR) in late pachytene germ cells and that MPK-1-dependent CEP-1 activation may be in part direct, as these two proteins interact in a yeast two-hybrid assay. In summary, we report our novel finding that MAP kinase signaling controls CEP-1-dependent apoptosis by several different pathways that converge on CEP-1. Since apoptosis is also restricted to pachytene stage cells in mammalian germlines, analogous mechanisms regulating p53 family members are likely to be conserved throughout evolution. | - |
dc.identifier.bibliographicCitation | PLOS GENETICS, v.7, no.8, pp.e1002238 | - |
dc.identifier.doi | 10.1371/journal.pgen.1002238 | - |
dc.identifier.issn | 1553-7404 | - |
dc.identifier.scopusid | 2-s2.0-80052332372 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30995 | - |
dc.identifier.url | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1002238 | - |
dc.identifier.wosid | 000294297000033 | - |
dc.language | 영어 | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Regulation of Caenorhabditis elegans p53/CEP-1-Dependent Germ Cell Apoptosis by Ras/MAPK Signaling | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | DAMAGE-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | SQUAMOUS CARCINOMA-CELLS | - |
dc.subject.keywordPlus | MAP KINASE PHOSPHATASE | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | CATALYTIC ACTIVATION | - |
dc.subject.keywordPlus | IONIZING-RADIATION | - |
dc.subject.keywordPlus | CHECKPOINT PROTEIN | - |
dc.subject.keywordPlus | MEIOTIC ARREST | - |
dc.subject.keywordPlus | P38 KINASE | - |
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