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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | U114 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 849 | - |
dc.citation.title | NATURE METHODS | - |
dc.citation.volume | 8 | - |
dc.contributor.author | Larance, Mark | - |
dc.contributor.author | Bailly, Aymeric P. | - |
dc.contributor.author | Pourkarimi, Ehsan | - |
dc.contributor.author | Hay, Ronald T. | - |
dc.contributor.author | Buchanan, Grant | - |
dc.contributor.author | Coulthurst, Sarah | - |
dc.contributor.author | Xirodimas, Dimitris P. | - |
dc.contributor.author | Gartner, Anton | - |
dc.contributor.author | Lamond, Angus I. | - |
dc.date.accessioned | 2023-12-22T05:42:33Z | - |
dc.date.available | 2023-12-22T05:42:33Z | - |
dc.date.created | 2020-01-30 | - |
dc.date.issued | 2011-10 | - |
dc.description.abstract | We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms. | - |
dc.identifier.bibliographicCitation | NATURE METHODS, v.8, no.10, pp.849 - U114 | - |
dc.identifier.doi | 10.1038/NMETH.1679 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.scopusid | 2-s2.0-80053363595 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30993 | - |
dc.identifier.url | https://www.nature.com/articles/nmeth.1679 | - |
dc.identifier.wosid | 000295358000021 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Stable-isotope labeling with amino acids in nematodes | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ELEGANS | - |
dc.subject.keywordPlus | SILAC | - |
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