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DC Field | Value | Language |
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dc.citation.endPage | 246 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 233 | - |
dc.citation.title | JOURNAL OF CELL BIOLOGY | - |
dc.citation.volume | 196 | - |
dc.contributor.author | Sonneville, Remi | - |
dc.contributor.author | Querenet, Matthieu | - |
dc.contributor.author | Craig, Ashley | - |
dc.contributor.author | Gartner, Anton | - |
dc.contributor.author | Blow, J. Julian | - |
dc.date.accessioned | 2023-12-22T05:36:33Z | - |
dc.date.available | 2023-12-22T05:36:33Z | - |
dc.date.created | 2020-01-30 | - |
dc.date.issued | 2012-01 | - |
dc.description.abstract | Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of Caenorhabditis elegans embryos. As expected, MCM-2-7 loading in late M phase depended on the prereplicative complex (pre-RC) proteins: origin recognition complex (ORC), CDC-6, and CDT-1. However, many features we observed have not been described before: GFP-ORC-1 bound chromatin independently of ORC-2-5, and CDC-6 bound chromatin independently of ORC, whereas CDT-1 and MCM-2-7 DNA binding was interdependent. MCM-3 chromatin loading was irreversible, but CDC-6 and ORC turned over rapidly, consistent with ORC/CDC-6 loading multiple MCM-2-7 complexes. MCM-2-7 chromatin loading further reduced ORC and CDC-6 DNA binding. This dynamic behavior creates a feedback loop allowing ORC/CDC-6 to repeatedly load MCM-2-7 and distribute licensed origins along chromosomal DNA. During S phase, ORC and CDC-6 were excluded from nuclei, and DNA was overreplicated in export-defective cells. Thus, nucleocytoplasmic compartmentalization of licensing factors ensures that DNA replication occurs only once. | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELL BIOLOGY, v.196, no.2, pp.233 - 246 | - |
dc.identifier.doi | 10.1083/jcb.201110080 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.scopusid | 2-s2.0-84856735748 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30991 | - |
dc.identifier.url | https://rupress.org/jcb/article/196/2/233/36722/The-dynamics-of-replication-licensing-in-live | - |
dc.identifier.wosid | 000299753200008 | - |
dc.language | 영어 | - |
dc.publisher | ROCKEFELLER UNIV PRESS | - |
dc.title | The dynamics of replication licensing in live Caenorhabditis elegans embryos | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | ORIGIN RECOGNITION COMPLEX | - |
dc.subject.keywordPlus | EUKARYOTIC DNA-REPLICATION | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | CELL-CYCLE | - |
dc.subject.keywordPlus | MCM2-7 HELICASE | - |
dc.subject.keywordPlus | GEMININ HOMOLOG | - |
dc.subject.keywordPlus | ATP-HYDROLYSIS | - |
dc.subject.keywordPlus | RE-REPLICATION | - |
dc.subject.keywordPlus | S-PHASE | - |
dc.subject.keywordPlus | CHROMATIN | - |
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