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DC Field | Value | Language |
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dc.citation.number | 11 | - |
dc.citation.title | OPEN BIOLOGY | - |
dc.citation.volume | 3 | - |
dc.contributor.author | Akay, Alper | - |
dc.contributor.author | Craig, Ashley | - |
dc.contributor.author | Lehrbach, Nicolas | - |
dc.contributor.author | Larance, Mark | - |
dc.contributor.author | Pourkarimi, Ehsan | - |
dc.contributor.author | Wright, Jane E. | - |
dc.contributor.author | Lamond, Angus | - |
dc.contributor.author | Miska, Eric | - |
dc.contributor.author | Gartner, Anton | - |
dc.date.accessioned | 2023-12-22T03:12:52Z | - |
dc.date.available | 2023-12-22T03:12:52Z | - |
dc.date.created | 2020-01-30 | - |
dc.date.issued | 2013-11 | - |
dc.description.abstract | Messenger RNA translation is regulated by RNA-binding proteins and small non-coding RNAs called microRNAs. Even though we know the majority of RNA-binding proteins and microRNAs that regulate messenger RNA expression, evidence of interactions between the two remain elusive. The role of the RNA-binding protein GLD-1 as a translational repressor is well studied during Caenorhabditis elegans germline development and maintenance. Possible functions of GLD-1 during somatic development and the mechanism of how GLD-1 acts as a translational repressor are not known. Its human homologue, quaking (QKI), is essential for embryonic development. Here, we report that the RNA-binding protein GLD-1 in C. elegans affects multiple microRNA pathways and interacts with proteins required for microRNA function. Using genome-wide RNAi screening, we found that nhl-2 and vig-1, two known modulators of miRNA function, genetically interact with GLD-1. gld-1 mutations enhance multiple phenotypes conferred by mir-35 and let-7 family mutants during somatic development. We used stable isotope labelling with amino acids in cell culture to globally analyse the changes in the proteome conferred by let-7 and gld-1 during animal development. We identified the histone mRNA-binding protein CDL-1 to be, in part, responsible for the phenotypes observed in let-7 and gld-1 mutants. The link between GLD-1 and miRNA-mediated gene regulation is further supported by its biochemical interaction with ALG-1, CGH-1 and PAB-1, proteins implicated in miRNA regulation. Overall, we have uncovered genetic and biochemical interactions between GLD-1 and miRNA pathways. | - |
dc.identifier.bibliographicCitation | OPEN BIOLOGY, v.3, no.11 | - |
dc.identifier.doi | 10.1098/rsob.130151 | - |
dc.identifier.issn | 2046-2441 | - |
dc.identifier.scopusid | 2-s2.0-84897551094 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30984 | - |
dc.identifier.url | https://royalsocietypublishing.org/doi/10.1098/rsob.130151 | - |
dc.identifier.wosid | 000330307000004 | - |
dc.language | 영어 | - |
dc.publisher | ROYAL SOC | - |
dc.title | RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let-7 miRNA pathways in Caenorhabditis elegans | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Caenorhabditis elegans | - |
dc.subject.keywordAuthor | miRNA | - |
dc.subject.keywordAuthor | gld-1 | - |
dc.subject.keywordAuthor | let-7 | - |
dc.subject.keywordAuthor | SILAC | - |
dc.subject.keywordPlus | DAMAGE-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | HISTONE GENE-EXPRESSION | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | TRANSLATIONAL REPRESSION | - |
dc.subject.keywordPlus | MICRORNA FAMILY | - |
dc.subject.keywordPlus | GERMLINE DEVELOPMENT | - |
dc.subject.keywordPlus | STAR PROTEIN | - |
dc.subject.keywordPlus | GLD-1 | - |
dc.subject.keywordPlus | TARGET | - |
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