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Lee, Ja Yil
School of Life Sciences
Research Interests
  • Investigated eukaryotic homologous recombination.

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Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

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Title
Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
Author
Kang, WooyoungHa, Kook SunUhm, HeesooPark, KyuhyongLee, Ja YilHohng, SungchulKang, Changwon
Issue Date
2020-01
Publisher
Nature Publishing Group
Citation
NATURE COMMUNICATIONS, v.11, no.1, pp.450
Abstract
Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.
URI
https://scholarworks.unist.ac.kr/handle/201301/30944
URL
https://www.nature.com/articles/s41467-019-14200-3
DOI
10.1038/s41467-019-14200-3
ISSN
2041-1723
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