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Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Rehydration of Polymeric, Aqueous, Biphasic System Facilitates High Throughput Cell Exclusion Patterning for Cell Migration Studies

Cited 10 times inthomson ciCited 6 times inthomson ci
Title
Rehydration of Polymeric, Aqueous, Biphasic System Facilitates High Throughput Cell Exclusion Patterning for Cell Migration Studies
Author
Tavana, HosseinKaylan, KerimBersano-Begey, TommasoLuker, Kathryn E.Luker, Gary D.Takayama, Shuichi
Keywords
96-Well plate; Aqueous phase; Aqueous two phase system; Biphasic systems; Cancer cells; Cell migration; Cell monolayers; cell-exclusion patterning; Cytoskeletons; Gap healing; High throughput; Interfacial tensions; Liquid handlers; Patterning methods; Phase droplets; Straight-forward method; Two phase systems; Washing-out
Issue Date
201108
Publisher
WILEY-V C H VERLAG GMBH
Citation
ADVANCED FUNCTIONAL MATERIALS, v.21, no.15, pp.2920 - 2926
Abstract
This paper describes a cell-exclusion patterning method facilitated by a polymeric aqueous two-phase system. The immersion aqueous phase (polyethylene glycol) containing cells rehydrates a dried disk of the denser phase (dextran) on the substrate to form a dextran droplet. With the right properties of the phase-forming polymers, the rehydrating droplet remains immiscible with the immersion phase. Proper formulation of the two-phase system ensures that the interfacial tension between the rehydrating droplet and the surrounding aqueous phase prevents cells from crossing the interface so that cells only adhere to the regions of the substrate around the dextran phase droplet. Washing out the patterning two-phase reagents reveals a cell monolayer containing a well-defined circular gap that serves as the migration niche for cells of the monolayer. Migration of cells into the cell-excluded area is readily visualized and quantified over time. A 96-well plate format of this "gap healing" migration assay demonstrates the ability to detect inhibition of cell migration by known cytoskeleton targeting agents. This straightforward method, which only requires a conventional liquid handler and readily prepared polymer solutions, opens new opportunities for high throughput cell migration assays
URI
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DOI
http://dx.doi.org/10.1002/adfm.201002559
ISSN
1616-301X
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