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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

Cited 18 times inthomson ciCited 13 times inthomson ci
Title
384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids
Author
Takayama, ShuichiHsiao, Amy Y.Tung, Yi-ChungQu, XiangguiPatel, Lalit R.Pienta, Kenneth J.
Keywords
3D; Anticancer drug; Cell types; Cell-based; Cellular model; Coculture; Culture methods; High-throughput; High-throughput screening; Sequential addition; Spheroid; Z-factor
Issue Date
2012-05
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.109, no.5, pp.1293 - 1304
Abstract
We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through Z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types
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DOI
10.1002/bit.24399
ISSN
0006-3592
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SLS_Journal Papers
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