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Rhee, Hyun-Woo
Biomimetic Chemistry Lab
Research Interests
  • Site-Specific Protein Labeling in Living Cells

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Microfluidic bead-based sensing platform for monitoring kinase activity

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Title
Microfluidic bead-based sensing platform for monitoring kinase activity
Author
Lee, Seung HwanRhee, Hyun-Woovan Noort, DannyLee, Hong JaiPark, Hee HoShin, Ik-SooHong, Jong-InPark, Tai Hyun
Keywords
Chemosensor; Kinase assay; Microfluidic device; Protein kinase activity
Issue Date
2014-07
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.57, no., pp.1 - 9
Abstract
Protein kinases control cellular functions by regulating protein phosphorylation. Monitoring protein kinase activity is essential for medical diagnosis and drug screening. Here, we present a novel microfluidic device for performing simple and versatile protein kinase assays, which utilizes a microbead-based chemosensor. An automatic mix-and-measure technique was achieved using integrated pneumatic valves. After mixing each reagent for the kinase assay, the mixture was transferred to the sensing chamber. Then, phosphorylated and fluorescence-labeled peptides were captured and detected by the chemosensor. A fluorescence signal was observed depending on the presence of the kinase. Furthermore, activities of various kinases in the cell lysate and the inhibitory effect of specific chemicals on the kinases were monitored. These results indicate that chemosensor-based microfluidic chips can be developed as a versatile kinase assay system.
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DOI
10.1016/j.bios.2014.01.039
ISSN
0956-5663
Appears in Collections:
SNS_Journal Papers
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