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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 479 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 468 | - |
dc.citation.title | NATURE CELL BIOLOGY | - |
dc.citation.volume | 19 | - |
dc.contributor.author | Sonneville, Remi | - |
dc.contributor.author | Moreno, Sara Priego | - |
dc.contributor.author | Knebel, Axel | - |
dc.contributor.author | Johnson, Clare | - |
dc.contributor.author | Hastie, C. James | - |
dc.contributor.author | Gartner, Anton | - |
dc.contributor.author | Gambus, Agnieszka | - |
dc.contributor.author | Labib, Karim | - |
dc.date.accessioned | 2023-12-21T22:17:56Z | - |
dc.date.available | 2023-12-21T22:17:56Z | - |
dc.date.created | 2019-09-10 | - |
dc.date.issued | 2017-04 | - |
dc.description.abstract | Replisome disassembly is the final step of DNA replication in eukaryotes, involving the ubiquitylation and CDC48-dependent dissolution of the CMG helicase (CDC45-MCM-GINS). Using Caenorhabditis elegans early embryos and Xenopus laevis egg extracts, we show that the E3 ligase CUL-2(LRR-1) associates with the replisome and drives ubiquitylation and disassembly of CMG, together with the CDC-48 cofactors UFD-1 and NPL-4. Removal of CMG from chromatin in frog egg extracts requires CUL2 neddylation, and our data identify chromatin recruitment of CUL2(LRR1) as a key regulated step during DNA replication termination. Interestingly, however, CMG persists on chromatin until prophase in worms that lack CUL-2(LRR-1), but is then removed by a mitotic pathway that requires the CDC-48 cofactor UBXN-3, orthologous to the human tumour suppressor FAF1. Partial inactivation of lrr-1 and ubxn-3 leads to synthetic lethality, suggesting future approaches by which a deeper understanding of CMG disassembly in metazoa could be exploited therapeutically. | - |
dc.identifier.bibliographicCitation | NATURE CELL BIOLOGY, v.19, no.5, pp.468 - 479 | - |
dc.identifier.doi | 10.1038/ncb3500 | - |
dc.identifier.issn | 1465-7392 | - |
dc.identifier.scopusid | 2-s2.0-85016334566 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/27443 | - |
dc.identifier.url | https://www.nature.com/articles/ncb3500 | - |
dc.identifier.wosid | 000400376100011 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | CUL-2(LRR-1) and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | XENOPUS EGG EXTRACTS | - |
dc.subject.keywordPlus | CELL-CYCLE PROGRESSION | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | EUKARYOTIC REPLISOME | - |
dc.subject.keywordPlus | C. ELEGANS | - |
dc.subject.keywordPlus | AAA-ATPASE | - |
dc.subject.keywordPlus | PROTEIN-DEGRADATION | - |
dc.subject.keywordPlus | FORK PROGRESSION | - |
dc.subject.keywordPlus | MCM2-7 HELICASE | - |
dc.subject.keywordPlus | FACTOR-1 FAF1 | - |
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