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DC Field | Value | Language |
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dc.citation.startPage | 4314 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 7 | - |
dc.contributor.author | Offenburger, Sarah-Lena | - |
dc.contributor.author | Bensaddek, Dalila | - |
dc.contributor.author | Murillo, Alejandro Brenes | - |
dc.contributor.author | Lamond, Angus I. | - |
dc.contributor.author | Gartner, Anton | - |
dc.date.accessioned | 2023-12-21T22:09:29Z | - |
dc.date.available | 2023-12-21T22:09:29Z | - |
dc.date.created | 2019-09-10 | - |
dc.date.issued | 2017-06 | - |
dc.description.abstract | Asymmetric cell divisions are required for cellular diversity and defects can lead to altered daughter cell fates and numbers. In a genetic screen for C. elegans mutants with defects in dopaminergic head neuron specification or differentiation, we isolated a new allele of the transcription factor HAM-1 [HSN (Hermaphrodite-Specific Neurons) Abnormal Migration]. Loss of both HAM-1 and its target, the kinase PIG-1 [PAR-1(I)-like Gene], leads to abnormal dopaminergic head neuron numbers. We identified discrete genetic relationships between ham-1, pig-1 and apoptosis pathway genes in dopaminergic head neurons. We used an unbiased, quantitative mass spectrometry-based proteomics approach to characterise direct and indirect protein targets and pathways that mediate the effects of PIG-1 kinase loss in C. elegans embryos. Proteins showing changes in either abundance, or phosphorylation levels, between wild-type and pig-1 mutant embryos are predominantly connected with processes including cell cycle, asymmetric cell division, apoptosis and actomyosin-regulation. Several of these proteins play important roles in C. elegans development. Our data provide an in-depth characterisation of the C. elegans wild-type embryo proteome and phosphoproteome and can be explored via the Encyclopedia of Proteome Dynamics (EPD) - an open access, searchable online database. | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.7, pp.4314 | - |
dc.identifier.doi | 10.1038/s41598-017-04375-4 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.scopusid | 2-s2.0-85021685281 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/27442 | - |
dc.identifier.url | https://www.nature.com/articles/s41598-017-04375-4 | - |
dc.identifier.wosid | 000404268900002 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | LEUCINE-ZIPPER KINASE | - |
dc.subject.keywordPlus | HYDROPHILIC-INTERACTION CHROMATOGRAPHY | - |
dc.subject.keywordPlus | ASYMMETRIC CELL-DIVISION | - |
dc.subject.keywordPlus | PROTEIN-PHOSPHORYLATION | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | FUNCTIONAL-ANALYSIS | - |
dc.subject.keywordPlus | MELK | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | NEMATODE | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
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