Full metadata record
DC Field | Value | Language |
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dc.citation.endPage | 8347 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 8337 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 47 | - |
dc.contributor.author | Cheon, Na Young | - |
dc.contributor.author | Kim, Hyun-Suk | - |
dc.contributor.author | Yeo, Jung-Eun | - |
dc.contributor.author | Scharer, Orlando D. | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.date.accessioned | 2023-12-21T18:45:39Z | - |
dc.date.available | 2023-12-21T18:45:39Z | - |
dc.date.created | 2019-08-26 | - |
dc.date.issued | 2019-09 | - |
dc.description.abstract | DNA repair is critical for maintaining genomic integrity. Finding DNA lesions initiates the entire repair process. In human nucleotide excision repair (NER), XPC-RAD23B recognizes DNA lesions and recruits downstream factors. Although previous studies revealed the molecular features of damage identification by the yeast orthologs Rad4-Rad23, the dynamic mechanisms by which human XPC-RAD23B recognizes DNA defects have remained elusive. Here, we directly visualized the motion of XPC-RAD23B on undamaged and lesion-containing DNA using high-throughput single-molecule imaging. We observed three types of one-dimensional motion of XPC-RAD23B along DNA: diffusive, immobile and constrained. We found that consecutive AT-tracks led to increase in proteins with constrained motion. The diffusion coefficient dramatically increased according to ionic strength, suggesting that XPC-RAD23B diffuses along DNA via hopping, allowing XPC-RAD23B to bypass protein obstacles during the search for DNA damage. We also examined how XPC-RAD23B identifies cyclobutane pyrimidine dimers (CPDs) during diffusion. XPC-RAD23B makes futile attempts to bind to CPDs, consistent with low CPD recognition efficiency. Moreover, XPC-RAD23B binds CPDs in biphasic states, stable for lesion recognition and transient for lesion interrogation. Taken together, our results provide new insight into how XPC-RAD23B searches for DNA lesions in billions of base pairs in human genome. | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.47, no.16, pp.8337 - 8347 | - |
dc.identifier.doi | 10.1093/nar/gkz629 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-85073310181 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/27336 | - |
dc.identifier.url | https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz629/5542879 | - |
dc.identifier.wosid | 000490576900007 | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | Single-molecule visualization reveals the damage search mechanism for the human NER protein XPC-RAD23B | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCISION-REPAIR | - |
dc.subject.keywordPlus | DIFFUSION-DRIVEN MECHANISMS | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DUAL INCISION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | XPC | - |
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