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Choi, Ung-Kyu
School of Nano-Bioscience and Chemical Engineering
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Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Staphylococcus aureus Using Evolutionary Operation Factorial Design Technique

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Title
Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Staphylococcus aureus Using Evolutionary Operation Factorial Design Technique
Author
Kim, Dae-HyunKim, Young-ChanChoi, Ung-Kyu
Keywords
Perilla frutescens var. acuta leaf; EVOP-factorial design technique; antibacterial activity; Staphylococcus aureus ATCC6538
Issue Date
2011-04
Publisher
MDPI AG
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.12, no.4, pp.2395 - 2407
Abstract
This study was undertaken to optimize extraction using evolutionary operation-factorial (EVOP) design technique to elicit the antibacterial activity of Perilla frutescens var. acuta leaf against Staphylococcus aureus ATCC6538. Higher antibacterial activity was achieved at higher extraction temperature and over a longer extraction time. Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent. The maximum antibacterial activity of ethanolic extract of P. frutescens leaf against S. aureus was obtained at 75 degrees C (R = -0.7904**) extraction temperature, 24 h (R = -0.7273**) extraction time, and 45% (R = -0.0635) ethanol concentration. The population of S. aureus was decreased from 7.535 log CFU/mL in the initial set to 4.865 log CFU/mL in the third set by EVOP factorial design technique, as well as to 2.600 log CFU/mL by extraction with ethyl acetate. Further, the ethyl acetate extract revealed the highest phenolic contents (111.3 +/- 8.6 mg% of dry sample) as compared to the other extracts. Also, the scanning electronic microscopic study of the ethanolic extract of P. frutescens revealed potential detrimental effect on the morphology of S. aureus.
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DOI
10.3390/ijms12042395
ISSN
1422-0067
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SLS_Journal Papers
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