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조승우

Cho, Seung Woo
Genome Engineering Lab.
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Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins

Author(s)
Cho, Seung WooLee, JihyunCarroll, DanaKim, Jin-SooLee, Junho
Issued Date
2013-11
DOI
10.1534/genetics.113.155853
URI
https://scholarworks.unist.ac.kr/handle/201301/25769
Fulltext
http://www.genetics.org/content/195/3/1177
Citation
GENETICS, v.195, no.3, pp.1177
Abstract
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F-1 progeny, demonstrating the high efficiency of this method.
Publisher
GENETICS SOC AM
ISSN
0016-6731

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