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Cho, Seung Woo
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Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins

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Title
Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins
Author
Cho, Seung WooLee, JihyunCarroll, DanaKim, Jin-SooLee, Junho
Issue Date
2013-11
Publisher
GENETICS SOC AM
Citation
GENETICS, v.195, no.3, pp.1177
Abstract
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F-1 progeny, demonstrating the high efficiency of this method.
URI
https://scholarworks.unist.ac.kr/handle/201301/25769
URL
http://www.genetics.org/content/195/3/1177
DOI
10.1534/genetics.113.155853
ISSN
0016-6731
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