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박성훈

Park, Sunghoon
Biochemical Engineering Lab.
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Production of 1,3-Propanediol from Glucose by Recombinant Escherichia coli BL21(DE3)

Author(s)
Lee, Jae HyeonLama, SumanKim, Jung RaePark, Sung Hoon
Issued Date
2018-03
DOI
10.1007/s12257-018-0017-y
URI
https://scholarworks.unist.ac.kr/handle/201301/25406
Fulltext
https://link.springer.com/article/10.1007%2Fs12257-018-0017-y
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.23, no.2, pp.250 - 258
Abstract
A range of recombinant strains of Escherichia coli were developed to produce 1,3-propanediol (1,3-PDO), an important C3 diol, from glucose. Two modules, the glycerol-producing pathway converting dihydroxyacetone phosphate to glycerol and the 1,3-PDO-producing pathway converting glycerol to 1,3-PDO, were introduced into E. coli. In addition, to avoid oxidative assimilation of the produced glycerol, glycerol oxidative pathway was deleted. Furthermore, to enhance the carbon flow to the Embden- Meyerhof-Parnas pathway, the Entner-Doudoroff pathway was disrupted by deleting 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Finally, the acetate production pathway was removed to minimize the production of acetate, a major and toxic by-product. Flask experiments were carried out to examine the performance of the developed recombinant E. coli. The best strain could produce 1,3-PDO with a yield of 0.47 mol/mol glucose. Along with 1,3-PDO, glycerol was produced with a yield of 0.33 mol/mol glucose.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
ISSN
1226-8372
Keyword (Author)
Escherichia coli BL21(DE3)glycerol1,3-PDOpathway engineering
Keyword
KLEBSIELLA-PNEUMONIAE J2B3-HYDROXYPROPIONIC ACIDCLOSTRIDIUM-BUTYRICUMGLYCEROLTRANSPORT

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