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- Park, Sunghoon
- Biochemical Engineering Lab.
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Enantioselective hydrolysis of racemic styrene oxide by epoxide hydrolase of Rhodosporidium kratochvilovae SYU-08
- Author(s)
- Lee, Ji Won, Lee, Eun Jung, Yoo, Seung Sik, Park, Sunghoon, Kim, Hee Sook, Lee, Eun Yeol
- Issued Date
- 2003-09
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.8, no.5, pp.306 - 308
- Abstract
- Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate, with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- ISSN
- 1226-8372
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