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박성훈

Park, Sunghoon
Biochemical Engineering Lab.
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Characterization of styrene catabolic genes of Pseudomonas putida SN1 and construction of a recombinant Escherichia coli containing styrene monooxygenase gene for the production of (S)-styrene oxide

Author(s)
Park, Mi SoBae, Jong WonHan, Ju HeeLee, Eun YeolLee, Sun-GuPark, Sunghoon
Issued Date
2006-07
URI
https://scholarworks.unist.ac.kr/handle/201301/25371
Fulltext
http://www.jmb.or.kr/journal/download.php?Filedir=../submission/Journal/016/&num=1265
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, no.7, pp.1041 - 1046
Abstract
Some Pseudomonas species can grow on styrene as a sole carbon and energy source. From the new isolate Pseudomonas putida SN1, the genes for styrene catabolism were cloned and sequenced. They were composed of four structural genes for styrene monooxygenase (styA and styB), styrene oxide isomerase (styC), and phenylacetaldehyde dehydrogenase (styD), along with two genes for the regulatory system (styS and styR). All the genes showed high DNA sequence (91% to 99%) and amino acid sequence (94% to 100%) similarities with the corresponding genes of the previously reported styrene-degrading Pseudomonas strains. A recombinant Escherichia coli to contain the styrene monooxygenase from the SN1 was constructed under the control of the T7 promoter for the production of enantiopure (S)-styrene oxide, which is an important chiral building block in organic synthesis. The recombinant E. coli could convert styrene into an enantiopure (S)-styrene oxide (ee > 99%) when induced by IPTG. The maximum activity was observed as 140 U/g cell, when induced with I mM IPTG at 15 degrees C.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
ISSN
1017-7825
Keyword (Author)
(S)-styrene oxidestyrene monooxygenasewhole-cell biocatalystPseudomonas putida SN1induction condition
Keyword
WHOLE-CELL BIOCATALYSTFUNCTIONAL-ANALYSISFLUORESCENS STSTRAIN VLB120DEGRADATIONHYDROXYLATIONEPOXIDESPROTEINS

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