File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

박성훈

Park, Sunghoon
Biochemical Engineering Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Glycerol assimilation and production of 1,3-propanediol by Citrobacter amalonaticus Y19

Author(s)
Ainala, Satish KumarAshok, SomasundarKo, YeounjooPark, Sunghoon
Issued Date
2013-06
DOI
10.1007/s00253-013-4726-z
URI
https://scholarworks.unist.ac.kr/handle/201301/25326
Fulltext
https://link.springer.com/article/10.1007%2Fs00253-013-4726-z
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.11, pp.5001 - 5011
Abstract
Citrobacter amalonaticus Y19 (Y19) was isolated because of its ability for carbon monoxide-dependent hydrogen production (water-gas shift reaction). This paper reports the assimilation of glycerol and the production of 1,3-propanediol (1,3-PDO) by Y19. Genome sequencing revealed that Y19 contained the genes for the utilization of glycerol and 1,2-propanediol (pdu operon) along with those for the synthesis of coenzyme B-12 (cob operon). On the other hand, it did not possess the genes for the fermentative metabolism of glycerol of Klebsiella pneumoniae, which consists of both the oxidative (dhaD and dhaK) and reductive (dhaB and dhaT) pathways. In shake-flask cultivation under aerobic conditions, Y19 could grow well with glycerol as the sole carbon source and produced 1,3-PDO. The level of 1,3-PDO production was improved when vitamin B-12 was added to the culture medium under aerobic conditions. Under anaerobic conditions, cell growth and 1,3-PDO production on glycerol was also possible, but only when an exogenous electron acceptor, such as nitrate or fumarate, was added. This is the first report of the glycerol metabolism and 1,3-PDO production by C. amalonaticus Y19.
Publisher
SPRINGER
ISSN
0175-7598
Keyword (Author)
Citrobacter amalonaticus Y19Glycerol metabolism1,3-PDOCoenzyme B-12
Keyword
ESCHERICHIA-COLIKLEBSIELLA-PNEUMONIAESALMONELLA-TYPHIMURIUMGENESCOBALAMINBACTERIASTRAINDEHYDROGENASEOPERON

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.