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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability


Reactive oxygen species regulate context-dependent inhibition of NFAT5 target genes

DC Field Value Language Kim, Nam-Hoon ko Hong, Bong-Ki ko Choi, Soo Youn ko Kwon, H. Moo ko Cho, Chul-Soo ko Yi, Eugene C ko Kim, Wan-Uk ko 2014-04-09T08:23:08Z - 2013-09-06 ko 2013-07 -
dc.identifier.citation EXPERIMENTAL AND MOLECULAR MEDICINE, v.45, no., pp.e32 - ko
dc.identifier.issn 1226-3613 ko
dc.identifier.uri -
dc.identifier.uri ko
dc.description.abstract The activation of nuclear factor of activated T cells 5(NFAT5), a well-known osmoprotective factor, can be induced by isotonic stimuli, such as activated Toll-like receptors (TLRs). It is unclear, however, how NFAT5 discriminates between isotonic and hypertonic stimuli. In this study we identified a novel context-dependent suppression of NFAT5 target gene expression in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS) or a high salt (NaCl) concentration. Although LPS and NaCl both used NFAT5 as a core transcription factor, these stimuli mutually inhibited distinct sets of NFAT5 targets within the cells. Although reactive oxygen species (ROS) are essential for this inhibition, the source of ROS differed depending on the context: mitochondria for high salt and xanthine oxidase for TLRs. Specifically, the high salt-induced suppression of interleukin-6 (IL-6) production was mediated through the ROS-induced inhibition of NFAT5 binding to the IL-6 promoter. The context-dependent inhibition of NFAT5 target gene expression was also confirmed in mouse spleen and kidney tissues that were cotreated with LPS and high salt. Taken together, our data suggest that ROS function as molecular sensors to discriminate between TLR ligation and osmotic stimuli in RAW 264.7 macrophages, directing NFAT5 activity toward proinflammatory or hypertonic responses in a context-dependent manner. ko
dc.description.statementofresponsibility open -
dc.language ENG ko
dc.subject Context dependency ko
dc.subject Hypertonicity ko
dc.subject Innate immunity ko
dc.subject NFAT5 ko
dc.subject Reactive oxygen species ko
dc.title Reactive oxygen species regulate context-dependent inhibition of NFAT5 target genes ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84882958162 ko
dc.identifier.wosid 000328122400003 ko
dc.type.rims ART ko
dc.description.wostc 3 *
dc.description.scopustc 0 * 2015-02-28 * 2014-07-12 *
dc.identifier.doi 10.1038/emm.2013.61 ko
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