We report a magnetic tunnel junction operating at room-temperature with organic magnetic semiconductor V[TCNE](x) (x similar to 2, TCNE: tetracyanoethylene) and Fe as the spin polarizer and analyzer while 10 mu rubrene layer serves as the tunnel barrier between them. At room-temperature, the magnetoresistance (MR) presents 16.7% of its peak value at 100K. We observed sign inversion of MR with increasing temperature, while the sign of the MR is independent of the polarity of the bias voltages. Our results suggest that V[TCNE](x) is a promising material for room-temperature spintronic applications.