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김동혁

Kim, Donghyuk
Systems Biology and Machine Learning Lab.
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Decoding genome-wide GadEWX-transcriptional regulatory networks reveals multifaceted cellular responses to acid stress in Escherichia coli

Author(s)
Seo, Sang WooKim, DonghyukO'Brien, Edward J.Szubin, RichardPalsson, Bernhard O.
Issued Date
2015-08
DOI
10.1038/ncomms8970
URI
https://scholarworks.unist.ac.kr/handle/201301/24285
Fulltext
https://www.nature.com/articles/ncomms8970
Citation
NATURE COMMUNICATIONS, v.6, pp.7970
Abstract
The regulators GadE, GadW and GadX (which we refer to as GadEWX) play a critical role in the transcriptional regulation of the glutamate-dependent acid resistance (GDAR) system in Escherichia coli K-12 MG1655. However, the genome-wide regulatory role of GadEWX is still unknown. Here we comprehensively reconstruct the genome-wide GadEWX transcriptional regulatory network and RpoS involvement in E. coli K-12 MG1655 under acidic stress. Integrative data analysis reveals that GadEWX regulons consist of 45 genes in 31 transcription units and 28 of these genes were associated with RpoS-binding sites. We demonstrate that GadEWX directly and coherently regulate several proton-generating/consuming enzymes with pairs of negative-feedback loops for pH homeostasis. In addition, GadEWX regulate genes with assorted functions, including molecular chaperones, acid resistance, stress response and other regulatory activities. These results show how GadEWX simultaneously coordinate many cellular processes to produce the overall response of E. coli to acid stress.
Publisher
NATURE PUBLISHING GROUP
ISSN
2041-1723
Keyword
RESISTANCE SYSTEMDECARBOXYLASE SYSTEMH-NSENTERIC BACTERIASMALL RNASLOW PHGADXGENEACTIVATORMECHANISMS

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