File Download

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

신태주

Shin, Tae Joo
Synchrotron Radiation Research Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Structural insight into molecular mechanism of poly (ethylene terephthalate) degradation

Author(s)
Joo, SeongjoonCho, In JinSeo, HogyunSon, Hyeoncheol FrancisSagong, Hye-YoungShin, Tae JooChoi, So YoungLee, Sang YupKim, Kyung-Jin
Issued Date
2018-01
DOI
10.1038/s41467-018-02881-1
URI
https://scholarworks.unist.ac.kr/handle/201301/23658
Fulltext
https://www.nature.com/articles/s41467-018-02881-1
Citation
NATURE COMMUNICATIONS, v.9, pp.382
Abstract
Plastics, including poly(ethylene terephthalate) (PET), possess many desirable characteristics and thus are widely used in daily life. However, non-biodegradability, once thought to be an advantage offered by plastics, is causing major environmental problem. Recently, a PET-degrading bacterium, Ideonella sakaiensis, was identified and suggested for possible use in degradation and/or recycling of PET. However, the molecular mechanism of PET degradation is not known. Here we report the crystal structure of I. sakaiensis PETase (IsPETase) at 1.5 angstrom resolution. IsPETase has a Ser-His-Asp catalytic triad at its active site and contains an optimal substrate binding site to accommodate four monohydroxyethyl terephthalate (MHET) moieties of PET. Based on structural and site-directed mutagenesis experiments, the detailed process of PET degradation into MHET, terephthalic acid, and ethylene glycol is suggested. Moreover, other PETase candidates potentially having high PET-degrading activities are suggested based on phylogenetic tree analysis of 69 PETase-like proteins.
Publisher
NATURE PUBLISHING GROUP
ISSN
2041-1723
Keyword
ALPHA/BETA-HYDROLASE FOLDPOLYETHYLENE TEREPHTHALATETHERMOBIFIDA-FUSCACUTINASEHYDROLYSISPOLY(ETHYLENE-TEREPHTHALATE)REPLACEMENTSEQUENCEDOCKINGSYSTEM

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.