Full metadata record
DC Field | Value | Language |
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dc.citation.number | 11 | - |
dc.citation.startPage | 2597 | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 17 | - |
dc.contributor.author | Devarakonda, Sivaranjani | - |
dc.contributor.author | Singh, Renu | - |
dc.contributor.author | Bhardwaj, Jyoti | - |
dc.contributor.author | Jang, Jaesung | - |
dc.date.accessioned | 2023-12-21T21:38:30Z | - |
dc.date.available | 2023-12-21T21:38:30Z | - |
dc.date.created | 2017-11-11 | - |
dc.date.issued | 2017-11 | - |
dc.description.abstract | Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30-40 spray coatings, corresponding to a 0.39-0.41 mg cm(-2) coating of nanoparticles on the paper and yielding a water contact angle of 150 degrees +/- 1 degrees. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 10(4) PFU mL(-1), and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL(-1), which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries. | - |
dc.identifier.bibliographicCitation | SENSORS, v.17, no.11, pp.2597 | - |
dc.identifier.doi | 10.3390/s17112597 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.scopusid | 2-s2.0-85034107775 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22929 | - |
dc.identifier.url | http://www.mdpi.com/1424-8220/17/11/2597 | - |
dc.identifier.wosid | 000416790500159 | - |
dc.language | 영어 | - |
dc.publisher | MDPI AG | - |
dc.title | Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical; Engineering, Electrical & Electronic; Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Chemistry; Engineering; Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | silica nanoparticles | - |
dc.subject.keywordAuthor | influenza virus | - |
dc.subject.keywordAuthor | paper sensor | - |
dc.subject.keywordAuthor | stencil printing | - |
dc.subject.keywordAuthor | electrochemical immunosensor | - |
dc.subject.keywordAuthor | label-free detection | - |
dc.subject.keywordAuthor | carbon nanotubes | - |
dc.subject.keywordPlus | LABEL-FREE DETECTION | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | IMMUNODEVICE | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | ELECTRODES | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | H1N1 | - |
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