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Jang, Jaesung
Sensors & Aerosols Lab.
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dc.citation.number 11 -
dc.citation.startPage 2597 -
dc.citation.title SENSORS -
dc.citation.volume 17 - Devarakonda, Sivaranjani - Singh, Renu - Bhardwaj, Jyoti - Jang, Jaesung - 2023-12-21T21:38:30Z - 2023-12-21T21:38:30Z - 2017-11-11 - 2017-11 -
dc.description.abstract Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30-40 spray coatings, corresponding to a 0.39-0.41 mg cm(-2) coating of nanoparticles on the paper and yielding a water contact angle of 150 degrees +/- 1 degrees. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 10(4) PFU mL(-1), and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL(-1), which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries. -
dc.identifier.bibliographicCitation SENSORS, v.17, no.11, pp.2597 -
dc.identifier.doi 10.3390/s17112597 -
dc.identifier.issn 1424-8220 -
dc.identifier.scopusid 2-s2.0-85034107775 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000416790500159 -
dc.language 영어 -
dc.publisher MDPI AG -
dc.title Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Chemistry, Analytical; Engineering, Electrical & Electronic; Instruments & Instrumentation -
dc.relation.journalResearchArea Chemistry; Engineering; Instruments & Instrumentation -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor silica nanoparticles -
dc.subject.keywordAuthor influenza virus -
dc.subject.keywordAuthor paper sensor -
dc.subject.keywordAuthor stencil printing -
dc.subject.keywordAuthor electrochemical immunosensor -
dc.subject.keywordAuthor label-free detection -
dc.subject.keywordAuthor carbon nanotubes -
dc.subject.keywordPlus LABEL-FREE DETECTION -
dc.subject.keywordPlus CARBON NANOTUBES -
dc.subject.keywordPlus RAPID DETECTION -
dc.subject.keywordPlus PLATFORM -
dc.subject.keywordPlus IMMUNODEVICE -
dc.subject.keywordPlus IMMUNOASSAY -
dc.subject.keywordPlus ELECTRODES -
dc.subject.keywordPlus BIOSENSOR -
dc.subject.keywordPlus H1N1 -


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