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Kim, Taesung
Microfluidics & Nanomechatronics Lab (μFNM)
Research Interests
  • Microfluidics & Nanofluidics
  • Nanoscale Transport Phenomena
  • MEMS & BioMEMS
  • Nanofabrication & Nanomechatronics

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Nanoscale hydrodynamic film for diffusive mass transport control in compartmentalized microfluidic chambers

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Title
Nanoscale hydrodynamic film for diffusive mass transport control in compartmentalized microfluidic chambers
Author
Kim, MinseokLim, Ji WonLee, Sung KukKim, Taesung
Issue Date
2017-10
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.89, no.19, pp.10286 - 10295
Abstract
A compartmentalized microfluidic chamber array that offers not only separate cell culture environments but also independent control of the diffusion of small molecules provides an extremely useful platform for cell cultivations and versatile cellular assays. However, it is challenging to incorporate both cell compartmentalization and active diffusion control in real-time and precise manners. Here, we present a novel nanoscale hydrodynamic film (NHF) that is formed between a solid substrate and a polydimethylsiloxane (PDMS) surface. The thickness of the NHF can be adjusted by varying the pressure applied between them so that the mass transfer through the NHF can also be controlled. These novel phenomena are characterized and applied to develop a compartmentalized microchamber array with diffusion-tunable and solution-switchable chemostat-like versatile bacterial assays. The NHF-based compartmentalization technique is ideal for not only continuous bacterial cultivation by consistently refreshing various nutrient sources but also various diffusion-based microbial assays such as chemical induction of synthetically engineered bacterial cells and selective growth of a specific bacterial strain with respect to chemical environments. In addition, we show that tight compartmentalization protects cells in the chambers, while biofilm formation and nutrient contamination are eliminated by loading a lysis buffer, which typically hinders long-term continuous cultures and accurate microbial assays on a chip. Therefore, we ensure that the NHF-based compartmentalization platform proposed in this work will facilitate not only fundamental studies in microbiology but also various practical applications of microbes for production of valuable metabolites and byproducts in a high-throughput and highly efficient format.
URI
https://scholarworks.unist.ac.kr/handle/201301/22842
URL
http://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b01966
DOI
10.1021/acs.analchem.7b01966
ISSN
0003-2700
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