The purpose of this research is to identify new service opportunities for enhancing driving safety of intra-city buses based on driving behavior analysis. Service opportunity identification involves finding target customers of service (to whom), motivations for service (why), service contents (what), and service delivery process (when, where). This paper presents an analysis of driving behaviors using the operational data of intra-city buses in conjunction with traffic accident data and drivers’ driving history data. This paper also presents four identified service opportunities based on the data analysis results. This research would contribute to enhancing driving safety of intra-city buses in Korea and serve as a basis for developing new services for driving safety enhancement.