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DC Field | Value | Language |
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dc.citation.endPage | 6609 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 6599 | - |
dc.citation.title | EMBO JOURNAL | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Barrett, Tracey E. | - |
dc.contributor.author | Scharer, Orlando D. | - |
dc.contributor.author | Savva, Renos | - |
dc.contributor.author | Brown, Tom | - |
dc.contributor.author | Jiricny, Josef | - |
dc.contributor.author | Verdine, Gregory L. | - |
dc.contributor.author | Pearl, Laurence H. | - |
dc.date.accessioned | 2023-12-22T12:09:15Z | - |
dc.date.available | 2023-12-22T12:09:15Z | - |
dc.date.created | 2017-01-26 | - |
dc.date.issued | 1999-12 | - |
dc.description.abstract | The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family of DNA glycosylases that initiate base-excision repair of G:U/T mismatches. Despite low sequence homology, the MUG/TDG enzymes are structurally related to the uracil-DNA glycosylase enzymes, but have a very different mechanism for substrate recognition. We have now determined the crystal structure of the Escherichia coli R-IUG enzyme complexed with an oligonucleotide containing a non-hydrolysable deoxyuridine analogue mismatched with guanine, providing the first structure of an intact substrate-nucleotide productively bound to a hydrolytic DNA glycosylase. The structure of this complex explains the preference for G:U over G:T mispairs, and reveals an essentially non-specific pyrimidine-binding pocket that allows MUG/TDG enzymes to excise the alkylated base, 3,N(4)-ethenocytosine, Together with structures for the free enzyme and for an abasic-DNA product complex, the MUG-substrate analogue complex reveals the conformational changes accompanying the catalytic cycle of substrate binding, base excision and product release. | - |
dc.identifier.bibliographicCitation | EMBO JOURNAL, v.18, no.23, pp.6599 - 6609 | - |
dc.identifier.doi | 10.1093/emboj/18.23.6599 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.scopusid | 2-s2.0-0345296914 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21295 | - |
dc.identifier.url | http://emboj.embopress.org/content/18/23/6599 | - |
dc.identifier.wosid | 000084177700002 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Crystal structure of a thwarted mismatch glycosylase DNA repair complex | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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