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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1969 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1961 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 33 | - |
dc.contributor.author | Gillet, Ludovic C.J. | - |
dc.contributor.author | Alzeer, Jawad | - |
dc.contributor.author | Scharer, Orlando D. | - |
dc.date.accessioned | 2023-12-22T10:38:56Z | - |
dc.date.available | 2023-12-22T10:38:56Z | - |
dc.date.created | 2017-01-26 | - |
dc.date.issued | 2005-01 | - |
dc.description.abstract | Aromatic amino and nitro compounds are potent carcinogens found in the environment that exert their toxic effects by reacting with DNA following metabolic activation. One important adduct is N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF), which has been extensively used in studies of the mechanisms of DNA repair and mutagenesis. Despite the importance of dG-AAF adducts in DNA, an efficient method for its incorporation into DNA using solid-phase synthesis is still missing. We report the development of a modified 'ultra-mild' DNA synthesis protocol that allows the incorporation of dG-AAF into oligonucleotides of any length accessible by solid-phase DNA synthesis with high efficiency and independent of sequence context. Key to this endeavor was the development of improved deprotection conditions (10% diisopropylamine in methanol supplemented with 0.25 M of beta-mercaptoethanol) designed to remove protecting groups of commercially available 'ultra-mild' phosphoramidite building blocks without compromising the integrity of the exquisitely base-labile acetyl group at N8 of dG-AAF. We demonstrate the suitability of these oligonucleotides in the nucleotide excision repair reaction. Our synthetic approach should facilitate comprehensive studies of the mechanisms of repair and mutagenesis induced by dG-AAF adducts in DNA and should be of general use for the incorporation of base-labile functionalities into DNA | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.33, no.6, pp.1961 - 1969 | - |
dc.identifier.doi | 10.1093/nar/gki335 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-17144362544 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21284 | - |
dc.identifier.url | https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gki335 | - |
dc.identifier.wosid | 000228398400034 | - |
dc.language | 영어 | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Site-specific incorporation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) into oligonucleotides using modified 'ultra-mild' DNA synthesis | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.description.journalRegisteredClass | scopus | - |
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