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Lee, Ja Yil
School of Life Sciences
Research Interests
  • Investigated eukaryotic homologous recombination.

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Direct observation of the formation of DNA triplexes by single-molecule FRET measurements

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Title
Direct observation of the formation of DNA triplexes by single-molecule FRET measurements
Author
Lee, Il BuemLee, Ja YilLee, Nam-KyungHong, Seok-Cheol
Keywords
DNA triplex;  H-DNA;  pH;  Single-molecule FRET;  Strand alignment
Issue Date
2012-07
Publisher
ELSEVIER SCIENCE BV
Citation
CURRENT APPLIED PHYSICS, v.12, no.4, pp.1027 - 1032
Abstract
In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH, ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors: at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly non-classical DNA structures, and provides insights into physical mechanism of such structures. (C) 2012 Elsevier B. V. All rights reserved
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DOI
10.1016/j.cap.2011.12.026
ISSN
1567-1739
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SLS_Journal Papers
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