Related Researcher

Author's Photo

Lee, Ja Yil
School of Life Sciences
Research Interests
  • Investigated eukaryotic homologous recombination.


Investigation of Protein-DNA Interactions by Using Single-molecule DNA Curtain Techniques

Cited 0 times inthomson ciCited 0 times inthomson ci
Investigation of Protein-DNA Interactions by Using Single-molecule DNA Curtain Techniques
Other Titles
단분자 디엔에이 커튼 기술을 이용한 단백질과 디엔에이의 상호작용 연구에 관한 고찰
Lee, Ja Yil
Issue Date
Korean Physical Society
PHYSICS & HIGH TECHNOLOGY, v.25, no.5, pp.32 - 36
Biological processes are accomplished by bio-molecular interactions. In particular, protein-DNA interactions are fundamental and essential for numerous biological phenomena. The study of protein-DNA interactions is very important for understanding the molecular mechanisms underlying diverse cellular activities. One of best ways to examine the interplay between proteins and DNA is directly viewing the biomolecules in real time. Such a direct observation seems not to be feasible when using traditional approaches in which numerous bio-molecules are simultaneously examined in bulk; hence, only the averaged propensity of the molecules is measured. However, single-molecule techniques, which overcome the ensemble-average effect in bulk assays, allow us to detect individual biomolecules and their reactions. Among the many single-molecule methods developed until now, the DNA curtain assay provides the most straightforward platform for the protein-DNA interaction. Here, the principles of operating the DNA curtain, as well as some biological issues to which this novel assay can be applied are introduced. The DNA curtain allows us to examine how proteins function on nucleic acid substrates by integrating lipid fluidity, microfluidics, nanofabrication, and fluorescence microscopy. Using the technique, we can examine 1) specific bindings of proteins, 2) protein movements such as diffusion or active translocation, and 3) collisions between proteins on double-stranded DNA substrates. Furthermore, the single-stranded DNA curtain facilitates studies on biological processes that are mediated by single-stranded DNA.
Go to Link
Appears in Collections:
SLS_Journal Papers
Files in This Item:
Investigation of Protein-DNA Interactions by Using Single-molecule DNA Curtain Techniques.pdf Download

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record


  • mendeley


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.