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Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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Thermostabilization of Candida antarctica lipase B by double immobilization:. Adsorption on a macroporous polyacrylate carrier and R1 silaffin-mediated biosilicification

Author(s)
Jun, ChanhaJeon, Byoung WookJoo, Jeong ChanQuang Anh Tuan LeGu, Sol-A.Byun, SungminCho, Dae HaengKim, DukkiSang, Byoung-InKim, Yong Hwan
Issued Date
2013-08
DOI
10.1016/j.procbio.2013.06.010
URI
https://scholarworks.unist.ac.kr/handle/201301/20355
Fulltext
http://www.sciencedirect.com/science/article/pii/S1359511313002808
Citation
PROCESS BIOCHEMISTRY, v.48, no.8, pp.1181 - 1187
Abstract
A large improvement in the thermostability of Candida antarctica lipase B (CALB) was achieved through double immobilization, i.e., physical adsorption and R1 silaffin-mediated biosilicification. The C-terminus of CALB was fused with the R1 silaffin peptide for biosilicification. The CALB-R1 fusion protein was adsorbed onto a macroporous polyacrylate carrier and then subsequently biosilicified with tetramethyl orthosilicate (TMOS). After R1 silaffin-mediated biosilicification, the double-immobilized CALB-R1 exhibited remarkable thermostability. The T-50(60) of the double-immobilized CALB-R1 increased dramatically from 45 to 72 degrees C and that was 27, 13.8, 9.8 and 9.9 degrees C higher than the T-50(60) values of free CALB-R1, CALB-R1 adsorbed onto a resin, commercial Novozym 435, and Novozym 435 treated with TMOS, respectively. In addition, the time required for the residual activity to be reduced to half (t(1/2)) of the double immobilized CALB-R1 elevated from 12.2 to 385 min, which is over 30 times longer life time compared free CALB-R1. The optimum pH for biosilicification was determined to be 5.0, and the double-immobilized enzyme showed much better reusability than the physically adsorbed enzyme even after 6 repeated reuses. This R1-mediated biosilicification approach for CALB thermostabilization is a good basis for the thermostabilization of industrial enzymes that are only minimally stabilized by protein engineering.
Publisher
ELSEVIER SCI LTD
ISSN
1359-5113
Keyword (Author)
Candida antarctica lipase BSilaffinR1 peptideBiosilicificationImmobilizationThermostability
Keyword
BACILLUS-CIRCULANS XYLANASEDIRECTED EVOLUTIONSILICA PARTICLESPICHIA-PASTORISENZYME-ACTIVITYENCAPSULATIONSTABILITYPROTEINDESIGN

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