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김용환

Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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Accelerated degradation of lignin by lignin peroxidase isozyme H8 (LiPH8) from Phanerochaete chrysosporium with engineered 4-O-methyltransferase from Clarkia breweri

Author(s)
Pham, Le Thanh MaiKim, Yong Hwan
Issued Date
2014-11
DOI
10.1016/j.enzmictec.2014.08.011
URI
https://scholarworks.unist.ac.kr/handle/201301/20339
Fulltext
http://www.sciencedirect.com/science/article/pii/S0141022914001550
Citation
ENZYME AND MICROBIAL TECHNOLOGY, v.66, pp.74 - 79
Abstract
Free-hydroxyl phenolic units can decrease or even abort the catalytic activity of lignin peroxidase HE during oxidation of veratryl alcohol and model lignin dimers, resulting in slow and inefficient lignin degradation. In this study we applied engineered 4-O-methyltransferase from Clarkia breweri to detoxify the inhibiting free-hydroxyl phenolic groups by converting them to methylated phenolic groups. The multistep, enzyme-catalyzed process that combines 4-O-methyltransferase and lignin peroxidase H8 suggested in this work can increase the efficiency of lignin-degradation. This study also suggests approaching the field of multi-enzyme in vitro systems to improve the understanding and development of plant biomass in biorefinery operations.
Publisher
ELSEVIER SCIENCE INC
ISSN
0141-0229

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