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DC Field | Value | Language |
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dc.citation.endPage | 84 | - |
dc.citation.startPage | 78 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 82 | - |
dc.contributor.author | Sharma, Abhinav | - |
dc.contributor.author | Han, Chang-Ho | - |
dc.contributor.author | Jang, Jaesung | - |
dc.date.accessioned | 2023-12-21T23:36:33Z | - |
dc.date.available | 2023-12-21T23:36:33Z | - |
dc.date.created | 2016-05-05 | - |
dc.date.issued | 2016-08 | - |
dc.description.abstract | Rapidity and high sensitivity are critical factors for the diagnoses of heart attacks, and cardiac troponin I (cTnI) is at present a clinical standard for its diagnosis. Here we report a rapid, label-free, and highly sensitive single-walled carbon nanotube (SWCNT) electrical immunosensor, featuring two pairs of electrodes. Two concentration electrodes (gaps: 25 and 80 μm) and two detection electrodes (source and drain; gap: 20 μm; width: 50 μm) were used for dielectrophoretic concentration of cTnI on the SWCNT channels and resistance measurements of the dielectrophoresis (DEP)-concentrated cTnI, respectively. The two concentration electrodes were imbedded between upper and lower dielectric layers, facing each other, underneath the -COOH-functionalized SWCNT channels deposited between the detection electrodes. Therefore, the gap between these imbedded concentration electrodes can be reduced to maximize the electric field intensity for DEP-mediated concentration of cTnI, thereby greatly reducing the detection time (1 min) and enhancing the limit of detection (0.7-0.8 pg mL- 1). Relative resistance changes of the SWCNTs were measured as cTnI concentration in Tris-Borate-EDTA (TBE; 0.0025×) and human serum diluted 500-fold with 0.0025× TBE decreased from 100 ng mL- 1 to 1 pg mL-1, and they were shown to be linear with the logarithm of cTnI concentration (R2=0.99 and 0.97, respectively). These immunosensors also showed high specificity over another cardiac biomarker, myoglobin, TBE medium (0.0025×), and 500-fold diluted human serum. The DEP-capture of cTnI depended on the frequency of the applied electric field, demonstrating the qualitative nature of the real part of the Clausius-Mossotti factor for cTnI. | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.82, pp.78 - 84 | - |
dc.identifier.doi | 10.1016/j.bios.2016.03.056 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.scopusid | 2-s2.0-84961927486 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/19142 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0956566316302470 | - |
dc.identifier.wosid | 000376545400013 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Rapid Electrical Immunoassay of the Cardiac Biomarker Troponin I through Dielectrophoretic Concentration using Imbedded Electrodes | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Cardiovascular disease | - |
dc.subject.keywordAuthor | Carbon nanotubes | - |
dc.subject.keywordAuthor | Cardiac troponin I | - |
dc.subject.keywordAuthor | Dielectrophoresis | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordPlus | MYOCARDIAL INJURY | - |
dc.subject.keywordPlus | PEPTIDE NANOTUBES | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | MYOGLOBIN | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | MANIPULATION | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | CHALLENGES | - |
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