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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Oct4-induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model

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Title
Oct4-induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model
Author
Kim, Jeong BeomLee, HyunahArauzo-Bravo, Marcos JHwang, KyujinNam, DonggyuPark, Myung RaeZaehres, HolmPark, Kook InLee, Seok-Jin
Issue Date
2015-12
Publisher
NATURE PUBLISHING GROUP
Citation
EMBO JOURNAL, v.34, no.20, pp.2483 - 2596
Abstract
The generation of patient-specific oligodendrocyte progenitor cells (OPCs) holds great potential as an expandable cell source for cell replacement therapy as well as drug screening in spinal cord injury or demyelinating diseases. Here, we demonstrate that induced OPCs (iOPCs) can be directly derived from adult mouse fibroblasts by Oct4-mediated direct reprogramming, using anchorage-independent growth to ensure high purity. Homogeneous iOPCs exhibit typical small-bipolar morphology, maintain their self-renewal capacity and OPC marker expression for more than 31 passages, share high similarity in the global gene expression profile to wild-type OPCs, and give rise to mature oligodendrocytes and astrocytes in vitro and in vivo. Notably, transplanted iOPCs contribute to functional recovery in a spinal cord injury (SCI) model without tumor formation. This study provides a simple strategy to generate functional self-renewing iOPCs and yields insights for the in-depth study of demyelination and regenerative medicine.
URI
https://scholarworks.unist.ac.kr/handle/201301/17699
URL
http://onlinelibrary.wiley.com/doi/10.15252/embj.201592652/abstract
DOI
10.15252/embj.201592652
ISSN
0261-4189
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BME_Journal Papers
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