BROWSE

Related Researcher

Author's Photo

Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

ITEM VIEW & DOWNLOAD

Production of Long-Chain alpha,omega-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
Production of Long-Chain alpha,omega-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils
Author
Sathesh-Prabu, ChandranLee, Sung Kuk
Issue Date
2015-09
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.63, no.37, pp.8199 - 8208
Abstract
Long-chain alpha,omega-dicarboxylic acids (LDCAs, >= C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated omega-oxidation pathway system with high omega-regioselectivity was heterologously expressed in Escherichia coli to produce DCAs from fatty acids. The resulting engineered E. coli produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered E. coli. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils
URI
https://scholarworks.unist.ac.kr/handle/201301/17564
URL
http://pubs.acs.org/doi/10.1021/acs.jafc.5b03833
DOI
10.1021/acs.jafc.5b03833
ISSN
0021-8561
Appears in Collections:
ECHE_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU