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Park, Lee Soon
School of Materials Science and Engineering
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  • Flexible OLED new materials, process and fabrication
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  • Nano-materials synthesis and application (Ag nano-wire and metal mesh electrode)

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Morphology and metabolism of Ba-alginate-encapsulated hepatocytes with galactosylated chitosan and poly(vinyl alcohol) as extracellular matrices

Cited 17 times inthomson ciCited 19 times inthomson ci
Title
Morphology and metabolism of Ba-alginate-encapsulated hepatocytes with galactosylated chitosan and poly(vinyl alcohol) as extracellular matrices
Author
Guo, XLYang, KSHyun, JYKim, WSLee, DHMin, KEPark, LSSeo, KHKim, YICho, CSKang, IK
Keywords
Alginate; Ammonia removal; Encapsulation; Galactosylated chitosan; Hepatocyte
Issue Date
2003-03
Publisher
TAYLOR & FRANCIS LTD
Citation
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v.14, no.6, pp.551 - 565
Abstract
Lactobionic acid, bearing a beta-galactose group, was coupled with chitosan to provide synthetic extracellular matrices together with poly(vinyl alcohol) (PVA). The hepatocytes encapsulated in Ba-alginate capsules with galactosylated chitosan (GC) and PVA as extracellular matrices showed aggregation morphologies as the incubation time increased. Ba-alginate-encapsulated hepatocytes with GC exhibited a higher metabolic function in albumin secretion compared to those entrapped in Ba-alginate beads and monolayer-cultured on a collagen-immobilized polystyrene dish. The ammonia removal ability of monolayer-cultured hepatocytes decreased with increasing culture time and disappeared completely after three days. In contrast, the ammonia removal ability of encapsulated and entrapped hepatocytes increased with increasing incubation time in the first seven and five days, respectively. Thereafter, the entrapped hepatocytes lost ammonia removal ability quickly while the encapsulated hepatocytes kept a relatively high ammonia removal ability up to 13 days. The trace amount of GC in the core matrices enabled encapsulated cells to enhance their ammonia removal and albumin secretion ability. The results obtained with 3-(3,4-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) also showed that the capsules incorporated with GC can provide a better microenvironment for cell aggregation along with nutrition and metabolite transfer. Due to the nature of the liquid core, the encapsulated hepatocytes showed very good mobility. This facilitated cell-cell interaction and cell-matrix interaction
URI
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DOI
10.1163/15685620360674245
ISSN
0920-5063
Appears in Collections:
MSE_Journal Papers
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