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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Cloning and characterization of cDNAs encoding V-H and V-L of a monoclonal anti-CEA antibody (CEA 79) cross-reactive with NCA-95 and generation of a single-chain Fv molecule (scFv)

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Title
Cloning and characterization of cDNAs encoding V-H and V-L of a monoclonal anti-CEA antibody (CEA 79) cross-reactive with NCA-95 and generation of a single-chain Fv molecule (scFv)
Author
Chung, JHChoi, SJHim, HJKim, IJChoi, IHLee, SDYi, KSSuh, Pann-GhillRyu, SHChung, HK
Keywords
carcinoembryonic antigen; CEA 79 scFv; cell adhesion molecule; immunoglobulin fragment; immunoglobulin heavy chain; immunoglobulin light chain; membrane protein; monoclonal antibody; tumor antigen
Issue Date
1997-12
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Citation
MOLECULES AND CELLS, v.7, no.6, pp.816 - 819
Abstract
We cloned complementary DNA (cDNA) encoding the variable regions of heavy chain (V-H) and of light chain (V-L,) of a monoclonal anti-carcinoembryonic antigen (CEA) antibody cross-reactive with nonspecific cross-reacting antigen-95 (NCA-95), which had been previously prepared and designated as CEA 79 (gamma(2a),,, kappa). From these cDNAs, a phagemid expression vector for the CEA79 single chain variable fragment (scFv) was generated. Enzyme-linked immunosorbent assay (ELISA), competitive ELISAs, and Western blotting confirmed that the scFv displayed on the surface of the bacteriophage had retained affinity for CEA and NCA-95. We then determined the nucleotide sequences of the cloned cDNAs for V-H, and V-L,. The sequence analysis revealed that V-H, and V-L, of the CEA 79 antibody represent new members of the mouse heavy chain subgroup "miscellaneous" and the kappa light chain subgroup "V", respectively
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ISSN
1016-8478
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BME_Journal Papers
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