Localization of two forms of phospholipase C-beta 1, a and b, in C6Bu-1 cells
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- Localization of two forms of phospholipase C-beta 1, a and b, in C6Bu-1 cells
- Bahk, Young Yil; Song, Hebok; Baek, Suk Hwan; Park, Byung Yu; Kim, Hyun; Ryu, Sung Ho; Suh, PG
- Issue Date
- Elsevier BV
- BIOCHIMICA ET BIOPHYSICA ACTA - LIPIDS AND LIPID METABOLISM, v.1389, no.1, pp.76 - 80
- Phospholipase C-beta 1 (PLC-beta 1), one of the PLC-beta isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-beta 1a) and 140 kDa (PLC-beta 1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene, In this study, the subcellular localization of the two phospholipases C-beta 1 proteins was examined in rat CGBu-1 glioma cells using immunological techniques, Immunoblot analysis revealed that the two forms of PLC-beta 1 were detectible in both cytosolic and nuclear fractions, PLC-beta 1a appeared to be located preferentially in the cytosol, whereas PLC-beta 1b was found predominantly in the nuclei of C6Bu-1 cells, Immunocytochemical experiments confirmed the differential localization of the two PLC-beta 1 species in C6Bu-1 cells. These results suggest that the two PLC-beta 1 proteins may have different physiological roles in the cell. (C) 1998 Elsevier Science B.V
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